In-situ magnetic alginate coated chitosan core@shell beads with excellent performance in simulated and real wastewater treatment: Behavior, mechanisms, and new perspectives.

Herein, a new hybrid magnetic core@shell biocomposite was prepared based on an alginate-bentonite core and a chitosan shell layer (mAB@Cs) where magnetic Fe3O4 NPs (50.7 nm) were in-situ generated on the surface via a simple non-thermal co-precipitation approach. The biocomposite has a high ability to magnetically separate and remove organic (ciprofloxacin (CPX)) and seven toxic inorganic (Cu2+, Cd2+, Co2+, Ni2+, Pb2+, Zn2+, and Hg2+) contaminants from simulated wastewater. Experimental results showed a CPX monolayer chemisorption with a Langmuir maximum adsorption capacity of 264.7 mg/g, maintained effectiveness up to the fifth cycle, and high removal rates of heavy metals ranging from 74.89 % to 99.86 % corresponding to adsorption capacities ranging from 12 to 20 mg/g. For a more accurate evaluation, the biocomposite was tested on a real urban wastewater sample (RWW) and it has manifested a noteworthy efficiency in removing a mixture of inorganic pollutants in terms of potassium K+ and orthophosphate phosphorous P-PO43-, and organic matter in terms of biological oxygen demand (BOD) and chemical oxygen demand (COD) with 46 %, 90 %, 84 %, and 64 % removal efficiencies, respectively. On top of this, a high inactivation rate of E. coli of the order of 96 % was recorded, making the prepared magnetic biocomposite adept for the simultaneous removal of emergent wastewater pollutants, from organic, inorganic, to pathogen microorganisms.

Porous polyvinyl fluoride coated cellulose beads for efficient removal of Cd(II) from phosphoric acid.

In order to enhance the removal of cadmium from phosphoric acid, it is imperative to explore novel resources that may be utilized for the development of highly effective and environmentally sustainable adsorbents. Cellulose beads are composed of naturally occurring polysaccharide fibers and find extensive utilization across several industrial sectors and applications. Within this framework, this research paper presents a green and simple method for producing porous cellulose beads using date palm fibers as the preferred raw material. The innovation lies in immersing the obtained cellulose beads in a Polyvinyl fluoride (PVDF)/N,N-dimethylformamide (DMF) suspension as a coating polymer with different concentrations (2.5, 5, 10 %) to maintain their stability in an acidic environment. The surface of cellulose/PVDF beads were subjected to multiple characterizations like Fourier transform infrared (FTIR) spectroscopy, Scanning electron microscopy (SEM), Thermogravimetric analysis (TGA), size distribution then pH stability confirming that the coating has been perfectly achieved and conserved well the shape of the beads. The coated cellulose/PVDF-2.5 % underwent evaluation by the process of batch adsorption experiments while different parameters were varied including contact time (5, 10, 20, 30, 60, 90 min), temperature (25, 35, 45 and 55 °C), and adsorbent mass (20, 40, 60, 80 and 100 mg). The obtained ICP data showed that the adsorption rate of Cd (II) from phosphoric acid medium decreased while increasing both temperature from 25 to 55 °C and contact time from 5 to 90 min while adding more adsorbent dosage from 20 to 100 mg enhanced the removal percentage. The cellulose/PVDF-2.5 % was more effective with an adsorption capacity equal to 3.4998 mg/g at optimal conditions including 25 °C as the temperature after 5 min as contact time and by adding a mass 100 mg of the biosorbent while the pH = 2 of the solution is maintained the same. The examined material's adsorption processes proved to be exothermic and non-spontaneous, and it proved that the pseudo-second-order model provided the best match for the cellulose/PVDF-2.5 % beads kinetics data. Furthermore, the cellulose beads exhibited exceptional reusability for up to four repeated cycles without undergoing desorption. The present study offers a viable approach for producing environmentally sustainable biomass-derived adsorbents. Additionally, the study validates the potential of cellulose/PVDF beads as an intriguing material for phosphoric acid decadmiation.

Cellulose beads supported CoFe2O4: A novel heterogeneous catalyst for efficient rhodamine B degradation via advanced oxidation processes.

In this study, a novel mechanical process was used to produce cellulose beads (CB). These beads were then doped with cobalt ferrite nanoparticles (CoFe2O4 NPs) to serve as catalysts for the degradation of rhodamine B (RhB) through peroxymonosulfate (PMS) activation. The physical and chemical properties of CoFe2O4 and CoFe2O4@CB catalysts were characterized using X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) combined with energy dispersive X-ray spectrometer (EDX), scanning transmission electron microscopy (STEM) techniques, and thermogravimetric analysis (TGA). To optimize RhB degradation efficiency, Response Surface Methodology (RSM) was employed, utilizing the Box-Behnken design (BBD). Under the optimized conditions of a catalyst dosage of 0.40 g/L, PMS dosage of 0.98 mM, RhB concentration of 40 mg/L, pH of 5.27, and reaction time of 60 min, a remarkable degradation efficiency of 98.51 % was achieved at a temperature of 25 °C. In quenching experiments, 1O2, SO4•-, and HO• species are produced in the CoFe2O4@CB/PMS system, with 1O2, and SO4•- species dominating RhB degradation. Remarkably, the new CoFe2O4@CB catalyst has demonstrated exceptional stability and reusability, validated by recycling tests (up to 78 % of RhB degradation efficiency after a 5-cycle experiment) and subsequent characterizations (FTIR, SEM, and EDX) emphasizing unchanged bands, uniform distribution, and consistent composition after reuse cycles. These results demonstrate the effectiveness of mechanically produced CoFe2O4@CB catalysts for advanced oxidation processes (AOPs), with promising applications in wastewater treatment.

Hemp cellulose nanocrystals for functional chitosan/polyvinyl alcohol-based films for food packaging applications.

Hemp is known for its swift growth and remarkable sustainability, requiring significantly less water, an adaptable cultivation to a wide range of climates when compared to other fibers sources, making it a practical and environmentally friendly choice for packaging materials. The current research seeks to extract cellulose nanocrystals (CNCs) from hemp fibers using alkali treatment followed by acid hydrolysis and assess their reinforcing capacity in polyvinyl alcohol (PVA) and chitosan (CS) films. AFM analysis confirmed the existence of elongated, uniquely nanosized CNC fibers. The length of the isolated CNCs was approximately 277.76 ± 61 nm, diameter was 6.38 ± 1.27 nm and its aspect ratio was 44.69 ± 11.08. The FTIR and SEM analysis indicated the successful removal of non-cellulosic compounds. Furthermore, the study explored the impact of adding CNCs at varying weight percentages (0, 0.5, 1, 2.5, and 5 wt%) as a strengthening agent on the chemical composition, structure, tensile characteristics, transparency, and water solubility of the bionanocomposite films. Adding CNCs to the CS/PVA film, up to 5 wt%, resulted in an improvement in both the Young's modulus and tensile strength of the bionanocomposite film, which are measured at (412.46 ± 10.49 MPa) and (18.60 ± 3.42 MPa), respectively, in contrast to the control films with values of (202.32 ± 22.50 MPa) and (13.72 ± 2.61 MPa), respectively. The scanning electron microscopy (SEM) images reveal the creation of a CS/PVA/CNC film that appears smooth, with no signs of clumping or clustering. The blending and introduction of CNCs have yielded transparent and biodegradable CS/PVA films. This incorporation has led to a reduction in the gas transmission rate (from 7.013 to 4.159 cm3 (m2 day·0.1 MPa))-1, a decrease in transparency (from 90.23% to 82.47%), and a lowered water solubility (from 48% to 33%). This study is the inaugural effort to propose the utilization of hemp-derived CNC as a strengthening component in the development of mechanically robust and transparent CS/PVA-CNC bio-nanocomposite films, holding substantial potential for application in the field of food packaging.

Development of shelf life-extending packaging for vitamin C syrup based on high-density polyethylene and extracted lignin argan shells.

Biobased packaging is an essential parameter in the pharmaceutical industry. In the present work, bio-composites consisting of high-density polyethylene (HDPE) as a matrix and lignin recovered from argan nut shells as filler were developed to investigate their potential use as packaging materials for vitamin C drugs. The lignin was extracted via alkali and klason processes, and the effects of the extraction method as well as the lignin content on the thermal, morphological, mechanical, and rheological properties of the produced composites, as well as their application for vitamin C packaging, were investigated. Among all the prepared packaging materials, the one with desirable results in pH, color stability, hardness, and mechanical characteristics was based on alkali lignin. It achieved its highest Young's modulus enhancement, 10.12 %, at 10 % alkali lignin loading, while the highest yield strain enhancement (4.65 %) was obtained with 2 % loading. When compared to neat HDPE and HDPE/klason lignin packaging materials, vitamin C solutions packed with this composite showed a lower oxidation rate, attributed to the extremely low pH variation and high color stability of the material, which decreased the rate of vitamin C degradation. According to these findings, HDPE/alkali lignin composite is a promising vitamin C syrup packaging material.

Chromium (III) adsorption from the phosphoric acid medium using DETA grafted Merrifield resin.

To selectively remove Cr (III) from synthetic phosphoric acid solution, a chelating ion exchanger was developed through Merrifield resin (MHL) functionalization with diethylenetriamine (DETA). The functional moieties of the grafted Merrifield resin were characterized and confirmed by means of Fourier-transform infrared spectroscopy. The morphological changes before and right after functionalization were visualized with Scanning electron microscopy and enhanced amine content was confirmed via energy dispersive X-ray. To assess the effectiveness of the MHL-DETA in the extraction of Cr (III) from a synthetic phosphoric acid solution, batch shaking adsorption tests were conducted through optimizing different factors such as contact time, metal ion concentration and temperature. According to our findings, higher adsorption was achieved when increasing contact time and decreasing metal ion concentration, while temperature variation doesn't affect much the process. The higher sorption yield was found to be 95.88% attained within 120 min at room temperature without varying the solution's pH. Under optimum conditions (120 min, 25 °C and 300 mg. L-1), the total sorption capacity was reported to be 38.35 mg. g-1. The system's adsorption behavior was found to be consistent with the Langmuir isotherm and the pseudo second-order model accurately described the kinetic data. In this view, Merrifield resin functionalized with DETA could be used as a promising adsorbent material for Cr (III) adsorption from synthetic phosphoric acid medium.

Stable smart packaging betalain-based from red prickly pear covalently linked into cellulose/alginate blend films.

Smart materials based on biomaterials have been shown growing interest by researchers. This paper investigated pH-indicator film with less leaching containing betalain molecule extracted from red prickly pear fixed in the cellulose-alginate blend as a matrix. Herein, the film was manufactured from a blend containing covalently bounded cellulose with betalain via the creation of a Fischer esterification (FE) to solve the leaching problem of dyes in contact with food. The structural, thermal, morphological optical, and mechanical properties and the pH-sensitive properties of films were examined. The FTIR and color analysis confirmed the fisher esterification. The fisher esterification led to a pH-indicator film with less leaching with significant color stability against UV light. The smart film changes colors with the pH values, where it goes from purple at a pH below 10 to yellow color at a pH above 10. All those proprieties with contact angles helped this film to be used as an intelligent film for monitoring salmon spoilage.

Intelligent food packaging film containing lignin and cellulose nanocrystals for shelf life extension of food.

The UV-protection films based on renewable materials extracted from natural sources are being one of the most interesting targets for the packaging industry. In this paper, the UV-protection films were produced based on modified lignin and cellulose nanocrystals, both were extracted from the Argania nutshell. The lignin was extracted via the alkylation process followed by chemical modification using epichlorohydrin. The chemical modification and the in-use temperature range of the films were investigated via FTIR and TGA, where the chemical modification of lignin improves the thermal property which is an essential parameter during the thermo-sealing of food packaging. On the other hand, the CNCs were extracted via acid hydrolysis treatment with the smallest size around 300 nm generating smooth texture of films. Finally, the produced films were shown a maximum absorption of the UV light in the range of 450-200 nm and great results in food packaging.

Bio-films based on alginate/modified clay through spray drying: Mechanical, rheological, morphological, and transport properties for potential use as active food packaging.

In this study, encapsulated modified montmorillonite with alginate (MMT-TBZC16/Alg) content and its incorporation into the biopolymer on morphological, mechanical, rheological, and transport properties of bio-based films based on chitosan were analyzed. The spray-drying approach was used to create organo-modified montmorillonite with alginate as a biopolymer. These encapsulating materials were then described and used as reinforcing agents for chitosan in the second step of this investigation to produce new reinforced biofilms with improved performance. This study aimed to study the effect of the addition of encapsulated organo-montmorillonite into the chitosan solution on the mechanical, rheological, and transport properties of the elaborated films. Films reinforced with encapsulated modified montmorillonite were characterized using Fourier transform infrared spectroscopy (FTIR), Water vapor transmission rate (WVTR) as well as mechanical and rheological properties. Adding encapsulated reinforcing agents to chitosan-based films is an option for improving mechanical and water barrier qualities. These results suggest that the developed MMT-TBZC16/Alginate-based chitosan films with higher performances could be used in designing potential packaging films.

Crosslinked starch-coated cellulosic papers as alternative food-packaging materials.

In general, during the papermaking process or the production of cellulosic materials for food-packaging applications, lignin and other amorphous components are usually removed via the pulping and multilevel bleaching process to entirely separate them from the fiber. The aim of this work was to study the positive effect that can impart the residual lignin remaining in the alkali-treated fiber surface over bleached fibers to produce an alternative food-packaging cellulosic paper. Herein, cellulosic papers based on alkali-treated and bleached fibers obtained from the Alfa plant were successfully prepared using a compression process. The as-obtained papers were coated by crosslinked starch using a solvent-casting method to improve their mechanical and surface properties. The morphological and contact angle results showed that the residual lignin in the alkali-treated cellulosic papers strongly increased the interfacial adhesion by making the structure denser and more compact, resulting in an improved water resistance property over the bleached ones. On the other hand, it also promoted char formation, slowing down the burning process, resulting in better flame resistance. Additionally, the mechanical properties demonstrated that the presence of lignin contributed to the material rigidity improvement without compromising its flexibility (folding endurance). The as-developed cellulosic papers coated with crosslinked starch could be used for the production of high-quality materials for food-packaging applications using conventional industrial processes.

Beneficiation of cactus fruit waste seeds for the production of cellulose nanostructures: Extraction and properties.

Cactus fruit waste seeds (CWS) are a by-product of the cactus fruit processing industry. Until now, CWS are not recoverable in any sector. The valorization of these residues may reduce their volume in the environment and transform them into valuable products. In this work, CWS have been identified for the first time as a sustainable lignocellulosic source. Cellulose microfibers (CMFs) and nanocrystals (CNCs) were successfully produced via alkali and bleaching treatments followed by sulfuric acid hydrolysis. It was found that the extracted CMFs showed an average diameter of 11 µm, crystallinity of 72%, and a yield of 25%. The as-produced CNCs exhibited a needle-like shape with a diameter of 13 ± 3 nm and length of 419 ± 48 nm, giving rise to an aspect ratio of 30.7, with a zeta potential value of - 30 mV and a charge content of sulfate groups of 287.8 mmol·kg-1. Herein, the obtained cellulosic derivatives with excellent properties from this underutilized waste can draw the attention of researchers towards CWS as a new type of biomass with virtually no hemicellulose, which could be of great interest to isolate and study the effects of how lignin interacts with cellulose.

Artemisia annua Stems a New Sustainable Source for Cellulosic Materials: Production and Characterization of Cellulose Microfibers and Nanocrystals.

In this study, Artemisia annua stem waste was identified, for the first time, as a potential natural source to produce cellulose microfibers (CMF), as well as cellulose nanocrystals (CNC) with unique functionalities by using various organic acids. The CMF extraction was carried out using alkali and bleaching treatments, while the CNC were isolated under acid hydrolysis by using sulfuric acid (S-CNC), phosphoric acid (P-CNC), and hydrochloric acid / citric acid mixture (C-CNC). The CMF and CNC physicochemical, structural, morphological, dimensional, and thermal properties were characterized. CMF with a yield of 53%, diameter of 5 to 30 µm and crystallinity of 57% were successfully obtained. In contrast, CNC showed a rod-like shape with an aspect ratio of 53, 95, and 64 and a crystallinity index of 84, 79, and 72% for S-CNC, P-CNC, and C-CNC, respectively. Results suggested that the type of acid significantly influenced the structure, morphology, and thermal stability of CNCs. Based on these results, Artemisia annua stem waste is a great candidate source for cellulose derivatives with excellent characteristics.

Manufacturing of macroporous cellulose monolith from green macroalgae and its application for wastewater treatment.

Enormous interest in using marine biomass as a sustainable resource for water treatment has been manifested over the past few decades. Herein, the objective was to investigate the possible use of green macroalgae (Codium tomentosum) for cellulose-based foam production through a versatile and convenient process. Macroporous cellulose monolith was prepared from cellulose hydrogel using freeze-drying process, resulting in a mechanically rigid monolith with a high swelling ratio. The as-produced spongy-like porous cellulosic material was used as bio-sorbent for wastewater treatment, particularly for removing methylene blue (MB) dye from concentrated aqueous solution. The adsorption capacity of MB was subsequently studied, and the effect of adsorption process parameters was determined in a controlled batch system. From the kinetic studies, it was found that the adsorption equilibrium was reached within 660 min. Furthermore, the analysis of the adsorption kinetics reveals that the data could be fitted by a pseudo-second order model, while the adsorption isotherm could be described by Langmuir isotherm model. The maximum adsorption capacity was found to be 454 mg/g. The findings suggested that the produced cellulose monolith could be used as a sustainable adsorbent for water treatment.

Photoluminescent biocomposite films of chitosan based on styrylbenzothiazolium-g-cellulose nanocrystal for anti-counterfeiting applications.

In the present investigation, novel photoluminescent and transparent biocomposite films based on chitosan reinforced with styrylbenzothiazolium-g-cellulose nanocrystal for anti-counterfeiting applications were successfully prepared by casting solvent. Three novel styrylbenzothiazolium derivatives were synthesized by Knoevenagel condensation and characterized by FTIR, 1H, 13C NMR and photoluminescence analysis. These photochromic compounds have been used to functionalize cellulose nanocrystal and the resulting fluorescent photonic materials were characterized by FTIR, 13C-CP/MAS NMR as well as photoluminescent analysis to confirm the successful grafting. It can be concluded that the addition of 5 wt% of fluorescent modified CNC to chitosan matrix increase the photoluminescent properties as well as improved the mechanical properties of the Cs/CNC-dye biocomposite films. These photoluminescent biocomposite film hold promising applicative value in anti-counterfeiting material in large-scale.

Recent progress on Ag/TiO2 photocatalysts: photocatalytic and bactericidal behaviors.

For many decades, titanium dioxide (TiO2) semiconductor has been extensively applied in several environmental applications due to its higher photocatalytic performances toward different organic pollutants, pharmaceutical compounds, and bacteria. However, its shortfall response to visible light, and the expeditious recombination rate of the photogenerated electron-hole pairs, hampers its utilization. Doping TiO2 semiconductor with silver nanoparticles is a sound strategy to (1) extend its photocatalytic activity to visible light, (2) prevent the electron/holes pairs recombination due to the formation of the Schottky barrier at the interfaces with TiO2 that act as an electron-trapping center, and (3) enhance its bactericide performances. This review focuses on the recent progress on silver-doped titanium dioxide (Ag/TiO2)-based photocatalysts. It addresses a wide range of Ag/TiO2 synthesis techniques, their physicochemical properties and discusses thoroughly the important role of silver (Ag) nanoparticles in enhancing the removal capacity and antibacterial performances of the Ag/TiO2 photocatalysts.

Efficient hybrid bionanocomposites based on iron-modified TiO2 for dye degradation via an adsorption-photocatalysis synergy under UV-Visible irradiations.

To overcome the titanium oxide limitations, Fe2O3- and Fe3O4-modified TiO2 (3:1) nanoparticles were synthesized by a humid and solid path, respectively. These nanoparticles were embedded in sodium alginate biopolymer to prepare beads with efficient adsorption and photocatalytic behaviors in cationic dye degradation under both UV and visible irradiations. Operating conditions were investigated such as initial methylene blue (MB) concentration and contact time to evaluate their impact on the process. The bead recycling was also scrutinized. We have come to the conclusion that Fe2O3-modified TiO2-Alg displayed superiorities, including expanded responsive wavelength range in the visible region (up to 700 nm), narrower band gap (1.79 eV), and better efficiency for MB removal in terms of adsorption capacities and photocatalytic effectiveness under both UV and visible irradiations. Furthermore, these beads can be effortlessly recovered from the reaction medium after the photocatalytic process and reused up to 5 cycles without any noteworthy decline in their initial properties.

Micro- and nano-structures of cellulose from eggplant plant (Solanum melongena L) agricultural residue.

Currently, agriculture sector produces enormous quantity of residues, creating severe environmental problems. These agricultural residues are rich in lignocellulosic fibers, making them sustainable sources to produce high added-value materials. This investigation aims to transform the eggplant plant residue (EPR) into purified cellulose microfibers (CMF) and cellulose nanocrystals (CNC). CMF with a yield of 54 %, diameter of 13.6 µm and crystallinity of 71 % were successfully obtained from raw EPR using alkali and bleaching treatments. By subjecting CMF to phosphoric and sulfuric acid hydrolysis, phosphorylated (P-CNC) and sulfated (S-CNC) were produced. P-CNC and S-CNC exhibited an aspect ratio of 89.4 and 74.2, zeta potential value of - 39.4 and - 28.7 mV, surface charge density of 116.7 and 218.2 mmol/kg cellulose and a crystallinity of 73 % and 80 %, respectively. Herein, the obtained cellulosic structures with excellent properties could be used in various applications, such as bio-derived fillers for polymer composites development.

Bioformulation of Microbial Fertilizer Based on Clay and Alginate Encapsulation.

This study aims to develop new formulations for microbial fertilizers Pseudomonas fluorescens Ms-01 (Pf) and Azosprillum brasilense DSM1690 (Ab) using two kinds of clay minerals. The studied formulations were prepared as hybrid materials based on halloysite and alginate [Ha-Ag] or montmorillonite and alginate polymers [Mt-Ag] and were applied to the bacterial strains to develop low cost, efficient, and slow-release capsules. Their efficiency was evaluated in comparison with alginate [Ag] as the control. The produced capsules were spherical in shape and were chemically and physically characterized and further analyzed for their swelling ratios, soil biodegradability, release kinetics of microbial cells, and their survival stability over 3 months of storage under different conditions (room temperature vs 4 °C). The effect of the capsules on the growth of wheat plants was also investigated. Results showed that both formulations were able to preserve bacterial survival which reached 14.8 log CFU g-1 after 3 months storage in the halloysite formulation. The swelling ratios were ranged between 61.5 ± 1.35% and 36.5 ± 5% for the montmorillonite and the halloysite formulations, respectively. The release kinetics revealed the slow-release capacity of the capsules mainly with the halloysite formulation which significantly released bacterial cells after 15 days of incubation in saline water (15.24 log CFU mL-1). The application of the capsules to wheat plants significantly increased root and shoot biomasses and nitrogen content in the roots. In conclusion, halloysite minerals seem to be more adapted as additive to alginate in microbial encapsulation.

Micro- and nano-celluloses derived from hemp stalks and their effect as polymer reinforcing materials.

Fiber-reinforced polymers have emerged as one of the most popular methods to improve the polymers' characteristics owing to their prominent properties. This study aimed to investigate the properties of cellulose microfibers (CMF), cellulose nanocrystals (CNC) and cellulose nanofibrils (CNF) extracted from hemp stalks, then their effect as reinforcement for the PVA-polymer. CMF have been extracted from hemp stalks with a diameter and yield of 16.96 µm and 63 %, respectively. Needle-shaped CNC were obtained from CMF using sulfuric acid hydrolysis at two hydrolysis times, while CNF exhibited a web-like structure obtained using TEMPO-oxidation followed by mechanical treatment. Cellulose derivatives were utilized to develop cellulose-based PVA composites; their transparency, chemical structure, thermal stability and mechanical properties were investigated. The incorporation of nanocellulose demonstrated a significant increase in mechanical properties compared to the neat PVA. The extracted nanocellulose could be used as nanofillers for the preparation of transparent and mechanically strong PVA-based nanocomposites.

Phosphoric acid-mediated green preparation of regenerated cellulose spheres and their use for all-cellulose cross-linked superabsorbent hydrogels.

With the growing environmental concerns and an emergent demand, a growing attention is turned to eco-friendly superabsorbent hydrogels instead of synthetic counterparts. Hydrogels based on cellulose derivatives can absorb and retain a huge amount of water in the interstitial sites of their structures, stimulating their uses in various useful industrial purposes. In this work, cross-linked superabsorbent composite hydrogel films (CHF) were designed, manufactured and characterized, by taking advantage of the combination of carboxymethyl cellulose (CMC), hydroxyethyl cellulose (HEC) and newly developed regenerated cellulose (RC) spheres. RC with sphere-like shape was successfully prepared using a green method based on cold phosphoric acid-mediated dissolution of microcrystalline cellulose (MCC) followed by regeneration process using water as anti-solvent. Prior to be used, the morphological and structural properties of RC spheres, with an average diameter of 477 ± 270 nm, were examined by SEM, AFM, XRD, FTIR and TGA techniques. CHF crosslinked with citric acid were, in fact, prepared by solvent casting method with different RC weight fractions (i.e. 0, 2.5, 5, 10 and 15 wt%), then the crosslinking reaction was triggered by thermal treatment at 80 °C during 8 h. Prepared CHF were then characterized in terms of their structural, thermal, tensile and transparency properties. Swelling tests were carried at three different aqueous media (i.e. with a pH = 3, 6.4 or 11) to evaluate the water retention capacity of hydrogel films, as well as, the pH effect on their swelling and hydrolytic degradation properties. Collected results reveal that CHF with low RC content (i.e. RC weight fraction of 2.5 or 5 wt%) have the best tensile and swelling properties, with a tensile strength and a swelling capacity (at pH = 6.4) up to 95 MPa and 4000%, respectively.